Cyclic AMP ELISA Kit 581001 from Cayman Chemical

Product Specs
ItemCyclic AMP ELISA Kit
CompanyCayman Chemical
Price Pricing Info Supplier Page View Company Product Page
Catalog Number581001
Quantity96 Well
ApplicationsELISA
Detection Range0.078-10 pmol/ml
DetectionColorimetric
MethodEIA
Sensitivity80% B/B0: 0.1 pmol/ml
Research AreacAMP Signaling, Cell Biology, Cell Signaling

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Cayman Chemical

Cayman Chemical Company 1180 East Ellsworth Road

Ann Arbor, Michigan 48108

United States

Phone: (800) 364-9897
(734) 971-3640

Company Profile

Website: http://www.caymanchem.com/

(1) Encystation stimuli sensing is mediated by adenylate cyclase AC2-dependent cAMP signaling in GiardiaNature CommunicationsNovember 9, 2023Han-Wei Shih, Germain C. M. Alas, Alexander R. Paredezwith ELISA buffer and loaded into 96-well plates provided by Cayman chemical (Cyclic AMP ELISA Kit, 581001, Cayman Chemicals). Briefly, the cAMP standard was generated with ELISA buffer. Both sample and standard

(2) Cyclic AMP mediates ovine cumulus–oocyte gap junctional function via balancing connexin 43 expression and phosphorylationEndocrine ConnectionsNovember 1, 2023Yufen Zhao, Erge Namei, Bingxue Yang, Xiangnan Bao, Wei Sun, Gerile Subudeng, Guifang Cao, Haijun Li, Gui WangcAMP concentrations in the CCs and oocytes were determined by ELISA using the cAMP ELISA kit (Cayman, 581001) according to the manufacturer’s protocol. Western blotting Immature COCs were cultured in standard

(3) GPR114/ADGRG5 is activated by its tethered peptide agonist because it is a cleaved adhesion GPCRJournal of Biological ChemistryOctober 1, 2023Tyler F. Bernadyn, Alexander Vizurraga, Rashmi Adhikari, Frank Kwarcinski, Gregory G. Tall10% FBS. Measurement of cAMP accumulation The cAMP ELISA assay kit from Cayman Chemical (Item No. 581001) was used to measure cAMP accumulation. Cells were collected and resuspended at a concentration of 1.0

(4) GPR65 inhibits human trophoblast cell adhesion through upregulation of MYLK and downregulation of fibronectin via cAMP-ERK signaling in a low pH environmentCell Communication and SignalingSeptember 18, 2023Jia Mao, Ying Feng, Yayun Zheng, Yaqiu Gao, Linyu Zhang, Xinrui Sun, Yilun Wu, Xiaofeng Zhu, Fang Main the cell supernatants were analyzed using an ELISA kit following the manufacturer's instructions (581001; Cayman). Briefly, the cell extract was added to a 96-well cover sheet, followed by the corresponding

(5) Methylxanthines Modulate Circadian Period Length Independently of the Action of PhosphodiesteraseMicrobiology SpectrumAugust 17, 2023Consuelo Olivares-Yañez, María P. Alessandri, Loreto Salas, Luis F. LarrondoIntracellular cAMP levels were determined using TCA extraction and a commercial ELISA kit (cat no. 581001, Cayman Chemical, Ann Arbor, MI, USA) according to the manufacturer’s recommendations. Supplementary

(6) Encystation stimuli sensing mediated by adenylate cyclase AC2-dependent cAMP signaling in GiardiaResearch SquareApril 26, 2023Alexander Paredez, Germain C.M. Shih, Alexander R. Paredezwith ELISA buffer and loaded into 96-well plates provided by Cayman chemical (Cyclic AMP ELISA Kit, 581001, Cayman Chemicals). Briefly, the cAMP standard was generated with ELISA buffer. Both sample and standard

(7) Encystation stimuli sensing mediated by adenylate cyclase AC2-dependent cAMP signaling in GiardiabioRxivApril 14, 2023Han-Wei Shih, Germain C.M. Alas, Alexander R. Paredezwith ELISA buffer and loaded into 96-well plates provided by Cayman chemical (Cyclic AMP ELISA Kit, 581001, Cayman Chemicals). Briefly, the cAMP standard was generated with ELISA buffer. Both sample and standard

(8) Differential IL18 signaling via IL18 receptor and Na-Cl co-transporter discriminating thermogenesis and glucose metabolism regulationNature communicationsDecember 8, 2022Zhang X, Luo S, Wang M, Cao Q, Zhang Z, Huang Q, Li J, Deng Z, Liu T, Liu CL, et al.0.1 M HCl and centrifuged at 1000 g for 10 min. cAMP level was determined using the cAMP ELISA Kit (581001, Cayman Chemical) according to manufacturers. Lipolysis Differentiated mature adipocytes were chased

(9) An approach for elucidating dermal fibroblast dedifferentiation in amphibian limb regenerationZoological LettersDecember 1, 2022Akira Satoh, Rena Kashimoto, Ayaka Ohashi, Saya Furukawa, Sakiya Yamamoto, Takeshi Inoue, Toshinori Hayashi, Kiyokazu Agatawas performed with 10 μg/ml for 2 h. ELISA ELISA was performed using Cayman cyclic AMP ELISA kit (#581001, Cayman Chemical, MI, USA). Samples were prepared from the Rolipram treated animals and control (DMSO

(10) Biotin Enhances Testosterone Production in Mice and Their Testis-Derived CellsNutrientsNovember 10, 2022Kota Shiozawa, Misato Maeda, Hsin-Jung Ho, Tomoko Katsurai, Md. Zakir Hossain Howlader, Kimiko Horiuchi, Yumi Sugita, Yusuke Ohsaki, Afifah Zahra Agista, Tomoko Goto, et al.was added for cell lysis. cAMP levels in the cell lysates were determined using a cAMP ELISA kit (#581001, Cayman Chemical). 2.6. Reporter Gene and RNA Interference Assays The CREB-mediated reporter plasmid

(11) Age-induced prostaglandin E(2) impairs mitochondrial fitness and increases mortality to influenza infectionNature communicationsNovember 9, 2022Chen J, Deng JC, Zemans RL, Bahmed K, Kosmider B, Zhang M, Peters-Golden M, Goldstein DR.were purchased from Invitrogen; albumin ELISA kits were purchased from Abcam (ab108792); cyclic AMP (581001) and PGE2 (514010) ELISA kits were purchased from Cayman Chemical; IFN-β ELISA (42410) kits were purchased

(12) Deficiency of WTAP in hepatocytes induces lipoatrophy and non-alcoholic steatohepatitis (NASH)Nature communicationsAugust 4, 2022Li X, Ding K, Li X, Yuan B, Wang Y, Yao Z, Wang S, Huang H, Xu B, Xie L, et al.manufacturer’s instructions, respectively. cAMP levels in eWAT were measured using cAMP ELISA kit (581001, Cayman). Liver glycogen levels were measured using Liver/Muscle glycogen assay kit (A043-1-1, Nanjing

(13) GIRK3 deletion facilitates kappa opioid signaling in chondrocytes, delays vascularization and promotes bone lengthening in miceBoneJune 1, 2022Earnest L. Taylor, Samantha R. Weaver, Ian M. Lorang, Katherine M. Arnold, Elizabeth W. Bradley, Ezequiel Marron Fernandez Marron Fernandez de Velasco, Kevin Wickman, Jennifer J. WestendorfcAMP was determined using acid extraction and a commercial ELISA per the supplier’s protocol (Cayman 581001). WT and Girk3−/− cells were plated at 1x105 cells per well in a 6-well plate and incubated with Dyn

(14) Angiotensin(1–7) activates MAS-1 and upregulates CFTR to promote insulin secretion in pancreatic β-cells: the association with type 2 diabetesEndocrine ConnectionsJanuary 11, 2022Xue-Lian Zhang et al.(3-isobutyl-1-methylxanthine, a phosphodiesterase inhibitor, 100 µM) for 15 min before the lysis buffer (Catalog# 581001, Cayman Chemical, Ann Arbor, MI, USA) was added. The lysate was collected 15 min later and centrifuged

(15) Skeletal muscle derived Musclin protects the heart during pathological overloadNature communicationsJanuary 10, 2022Szaroszyk M, Kattih B, Martin-Garrido A, Trogisch FA, Dittrich GM, Grund A, Abouissa A, Derlin K, Meier M, Holler T, et al.manufacturer’s protocol. For the measurement of cAMP levels using the cAMP enzyme immunoassay (EIA) kit (#581001, Cayman Chemical) the samples were processed as described above. The plasma Musclin concentration was

(16) Adenylyl cyclase 3 regulates osteocyte mechanotransduction and primary ciliumBiochemical and biophysical research communicationsOctober 8, 2021Duffy MP, Sup ME, Guo XE.supernatant collected. cAMP ELISA was performed according to manufacturer’s instructions (Cayman Chemical, 581001) and cAMP levels were normalized to sample protein concentration (ThermoScientific Pierce BCA Protein Assay Kit

(17) Ecto‑5'‑nucleotidase (CD73) inhibits dorsal root ganglion neuronal apoptosis by promoting the Ado/cAMP/PKA/CREB pathwayExperimental and Therapeutic MedicineSeptember 28, 2021Minghao Shao, Chaojun Zheng, Xiaosheng Ma, Feizhou Lyucontent was calculated according to the manufacturer's instructions. The cAMP detection kit (cat. no. 581001) was purchased from Cayman Chemical Company. The content of cAMP in the cells was detected by ELISA

(18) Adrenomedullin increases cAMP accumulation and BDNF expression in rat DRG and spinal motor neuronsIranian journal of basic medical sciencesJuly 1, 2021Sisakht M, Khoshdel Z, Mahmoodazdeh A, Shafiee SM, Takhshid MA.the positive controls. The cAMP level was measured by a specific ELISA kit (Cayman chemical, Item No. 581001) and in accordance with the manufacturer’s instructions. Quantification of phosphorylated-CREB (p-CREB)

(19) Transcriptional co-activator regulates melanocyte differentiation and oncogenesis by integrating cAMP and MAPK/ERK pathwaysCell reportsMay 18, 2021Ostojić J, Yoon YS, Sonntag T, Nguyen B, Vaughan JM, Shokhirev M, Montminy M.concentrations are indicated in Figures and Legends. Cellular cAMP levels were measured using an ELISA kit (581001, Cayman Chemical Company) according to manufacturer’s instructions. Immunofluorescence HEK293T and

(20) Aging-dependent mitochondrial dysfunction mediated by ceramide signaling inhibits antitumor T cell responseCell reportsMay 4, 2021Vaena S, Chakraborty P, Lee HG, Janneh AH, Kassir MF, Beeson G, Hedley Z, Yalcinkaya A, Sofi MH, Li H, et al.12–151 bcAMP,Sigma-Aldrich,Cat# B5386 ABC294640,RedHill Biopharm,Cat# ABC924640 cAMP Elisa Kit,Cayman Chemical,Cat# 581001 mDivi,Sigma-Aldrich,Cat#M0199 "Anti-rabbit Immuno-gold labeled, 1.4 nm","Nanoprobes, Inc.","#2004,

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Cyclic AMP ELISA Kit from Cayman Chemical

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Cayman Chemical

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Bench Tips for Optimizing ELISA: It All Comes Out in the Wash

Cyclic AMP ELISA Kit from Cayman Chemical

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